Peroxidaze Catalyzed singlet oxygen formation from hydrogen peroxide
نویسندگان
چکیده
منابع مشابه
Protoporphyrin IX catalyzed hydrogen peroxide to generate singlet oxygen.
AIM To study the role of protoporphyrin IX (pPIX) in mitochondrial metabolism of hydrogen peroxide (H2O2). METHODS O2 (-) specific fluorescent markers DMA (9,10-dimerthylanthracence) and SOSG (Singlet Oxygen Sensor Green reagent) were used for measurement of singlet oxygen ((1)O2). Catalyzing conversion of H2O2 into (1)O2 by pPIX was monitored in vitro under varied H2O2 content, temperature, ...
متن کاملAscorbate reacts with singlet oxygen to produce hydrogen peroxide.
Singlet oxygen is a highly reactive electrophilic species that reacts rapidly with electron-rich moieties, such as the double bonds of lipids, thiols, and ascorbate (AscH-). The reaction of ascorbate with singlet oxygen is rapid (k = 3 x 10(8) M(-1) s(-1)). Here we have investigated the stoichiometry of this reaction. Using electrodes to make simultaneous, real-time measurements of oxygen and h...
متن کاملSinglet oxygen production by chloroperoxidase-hydrogen peroxide-halide systems.
Singlet oxygen production in the chloroperoxidase-hydrogen peroxide-halide system was studied using 1268 nm chemiluminescence. With chloride or bromide ions, singlet oxygen is produced by the mechanism (formula; see text) (formula; see text) where X- is chloride or bromide ion. Under conditions where there is high enzyme activity and when Reaction B is fast relative to Reaction A, singlet oxyge...
متن کاملSinglet oxygen generation from poly[4-diacetoxyiodo]styrene and hydrogen peroxide
Treatment of hydrogen peroxide with a polymer-supported hypervalent iodine compound, poly[4-diacetoxyiodo] styrene (PDAIS), generates singlet molecular oxygen (O2) . Singlet oxygen generation was proved by trapping with typical organic compounds such as conjugated dienes, aromatic dienes, and electron-rich alkene. When compared to monomer analogue, the use of PDAIS in peroxidation of substrates...
متن کاملContrasting Effects of Singlet Oxygen and Hydrogen Peroxide on Bacterial Community Composition in a Humic Lake
Light excitation of humic matter generates reactive oxygen species (ROS) in surface waters of aquatic ecosystems. Abundant ROS generated in humic matter rich lakes include singlet oxygen ((1)O2) and hydrogen peroxide (H2O2). Because these ROS differ in half-life time and toxicity, we compared their effects on microbial activity ((14)C-Leucine incorporation) and bacterial community composition (...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80857-0